Study design

Type of tubes 

Number of tubes 

Respite 

Centrifugation 

Alliquotation 

Volume to be distributed 

Storage 

Vacuette 

Serum without gel, 6 mL 

8

30-120 min 

2200xg for 10 min at RT 

8 x 0,7 mL
6 x 0,5 mL

525 uL serum  

-80°C 

4 x 1,0 mL 

865 uL serum  

Vacuette EDTA, 3 mL 

 1 

2 x 1,0 mL 

865 uL full blood  

Vacuette 

EDTA, 6 mL 

5 

Max 2 hours 

 2200xg for 10 min at RT

6 x 0,7 mL 

525 uL plasma  

2 x 1,0 mL

864 uL plasma  

  2 x 2,0 mL  

1800 uL plasma  

1 x 1,0 mL

865 uL buffycoat 

PAXgene, 2,5 mL 

1 

3-72 hours at RT 

Intermediate storage at -20°C for 24 hours, then -80°C. 

Urine, 20 mL 

1 

5 x 2 mL 

1800 uL urine 

-80°C 

Type of tubes 

Number of tubes 

Respite 

Centrifugation 

Alliquotation 

Volume to be distributed 

Storage 

Vacuette 

Serum without gel, 10 mL 

2 

30-120 min 

1500xg for 25 min at RT 

6 x 0,5 mL 

3 mL serum 

-80°C 

4 x 2 mL 

6 mL serum 

Vacuette EDTA, 6 mL 

 1 

2 x 2 mL 

3 mL fullblood 

Vacuette 

EDTA, 9 mL 

5 

Max 2 hours 

2 tubes, 2200xg for 10 min at RT 

6 x 2 mL 

12 mL plasma (warm) 

1 tube (on ice), 2500 xg at 4°C 

6 x 0,5 mL 

3 mL plasma (cold) 

2 x 1 mL 

2 mL plasma (cold) 

1 x 0,5 mL 

0,5 mL buffycoat 

1 tube (on ice), 

 i.)  200xg for 20 min without brake at 4°C,  

ii.) 200xg for 10 min without brake at 4°C,  

iii)1000xg for 10 min at 4°C 

1 x 0,5 mL 

0,5 mL blood platelets 

CPT, 8 mL 

1 

i) 1500 xg for 25 min at RT 

ii) 1000xg for 10 min 

PAXgene, 2,5 mL 

1 

3-72 hours at RT 

Intermediate storage at -20°C for 24 hours, then -80°C. 

Urine, 20 mL 

1 

6 x 2 mL 

9 mL urine 

-80°C 

Name

Description 

EORTC-QLQ-C30 

EORTC-QLQ-BR23 

Quality-of-life questionnaire: consists of 53 questions about daily activities, as well as your physical and mental health.. 

The Mishel Uncertainty in Illness Scale (MUIS) 

4 statements assessing how information about the disease and treatment has been perceived and understood.

Hospital Anxiety and Depression Scale (HAD) 

14 questions, of which 7 assess anxiety and 7 assess depression.

Functional Assessment of Cancer Therapy-Fatigue (FACT-F) 

13 questions about fatigue/exhaustion during the past 7 days.

Functional Assessment of Cancer Therapy-Endocrine Scale (FACT-ES) 

56 questions about how the patients have been feeling over the past 7 days. The questions cover physical and psychological conditions as well as side effects of endocrine treatment.

Functional Assessment of Cancer Therapy-Breast (FACT-B) 

10 questions on quality of life in breast cancer patients.

Fatigue Severity Scale (FSS) 

9 questions about how one has experienced fatigue and exhaustion over the past 2 weeks.  

Fatigue Visual Analog Scale (fVAS) 

VAS is a line where the far left end corresponds to 0 = “no problems with fatigue and exhaustion,” and the far right end corresponds to 100 = “the worst possible fatigue and exhaustion.”

Food questionnaire

Questionnaire on whether participants avoid any of 36 different food items, with the response options “Yes,” “No,” and “Partially.” It also includes seven additional questions about the consumption of milk and dairy products, and one question about alcohol consumption.

Questions about joint pain 

3 questions about joint pain with yes/no response options, including grading of morning stiffness in the joints and any muscle pain.

Compliance 

A modified version of the 8-item Morisky Medication Adherence Scale (MMAS), which also includes 2 statements about “belief in the medication.”

Subjective Health Complaints

The questionnaire consists of 29 questions about subjective somatic and psychosocial complaints over the past 30 days. SHC has four subscales: musculoskeletal pain, “pseudoneurology,” gastrointestinal complaints, and allergy and common cold symptoms.

Others